Catalog Number : 500-M26
Produced in BALB/c mice using highly pure recombinant human TNF-α as the immunizing antigen. This antibody was purified from cell culture by Protein G affinity chromatography.
|Application:||ELISA, Western Blot, Neutralization, Immunohistochemistry|
Anti-Human TNF-α (BioGems Catalog #300-01A)
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentrations are 15.0-20.0 μg/ml overnight at 4˚C. An HRP-labeled polymer detection system was used with DAB Chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.
*Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.
To yield one-half maximal inhibition [ND50] of the biological activity of Human TNF-α (0.5 ng/ml), a concentration of < 0.1 μg/ml of this antibody is required.
In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect at least 1000 pg/ml of recombinant Human TNF-α when used with BioGems' biotinylated antigen affinity purified anti-Human TNF-α (60-325BT) as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml.
To detect Human TNF-α by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 µg/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human TNF-α is 0.5-1.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.