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Anti-Human TNF-α

Catalog Number : 500-P31AG


Produced from sera of goats pre-immunized with highly pure recombinant Human TNF-α. Anti-Human TNF-α specific antibody was purified by affinity chromatography employing immobilized Human TNF-α matrix.

Additional Information

Recombinant Human TNF-α (BioGems Catalog #300-01A)
Polyclonal Goat
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentrations are 2.5 μg/ml-5.0 μg/ml with a two-hour incubation at room temperature or an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. This antibody was also tested in an HRP-biotin labeling detection system. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary.
To yield one-half maximal inhibition [ND50] of the biological activity of Human TNF-α (0.25 ng/ml), a concentration of 0.012 - 0.014 µg/ml of this antibody is required.
Sandwich ELISA:
To detect Human TNF-α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human TNF-α (60-325GBT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human TNF-α.
Western Blot:
To detect Human TNF-α by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human TNF-α is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

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