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Anti-Human sTNF Receptor Type II



Catalog Number : 500-P168

Description

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hsTNF-receptor II. Anti-Human sTNF-receptor II specific antibody was purified by affinity chromatography employing immobilized hsTNF-receptor II matrix.

Additional Information

Immunogen
Recombinant Human sTNF Receptor Type II (BioGems Catalog #310-12)
 
Clone:
Polyclonal Rabbit
 
Format:
purified
 
Applications:
Immunohistochemistery:
This antibody stained PC-3 and CaCo-2 cells.  The primary antibody was incubated at 3.0 mg/ml overnight at 4°C followed by a fluorescent labeled secondary antibody.  Optimal concentrations and conditions may vary.  Information is courtesy of the Cell Profiling group, SciLifeLab Stockholm.
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Human sTNF Receptor-II (0.5 μg/ml) a concentration of 15.0 - 20.0 μg/ml of this antibody is required.
Sandwich ELISA:
To detect hsTNF-receptor II by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human sTNF-receptor II (60-328BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsTNF-receptor II.
Western Blot:
To detect hsTNF-receptor II by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsTNF-receptor II is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
 
Reactivity:
Human
 

Availability: In stock

Size Price Qty
50μg
$195.00
100μg
$250.00
1mg
$1,700.00