Catalog Number : 500-M02R
Produced in mice using highly pure recombinant Human soluble IL-2 Receptor α as the immunizing antigen. This IgG2aK antibody was purified from cell culture by Protein G affinity chromatography.
|Application:||ELISA, Western Blot, Immunohistochemistry|
Anti-Human sIL-2Rα (BioGems Catalog #200-02R)
This antibody stained frozen human tonsil. The recommended concentration is 5.0 μg/ml at room temperature. A labeled polymer detection system was used with DAB chromogen. Optimal concentrations and conditions may vary.
In a sandwich ELISA (assuming 100μl/well), a concentration of 4.0-8.0 μg/ml of this antibody will detect at least 100 pg/ml of recombinant Human sIL-2Rα when used in conjunction with compatible secondary reagents.
To detect Human sIL-2Rα by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human sIL-2Rα is 2.0-4.0 ng/lane, under non-reducing conditions.