Catalog Number : 500-M44
Produced in BALB/c x ICR F1 mice using highly pure (>98%) recombinant human SCF as the immunizing antigen. This IgG1K antibody was purified from ascites fluid by Protein A affinity chromatography.
|Application:||ELISA, Western Blot, Immunohistochemistry|
Anti-Human SCF (BioGems Catalog #300-07)
This antibody stained CACO-2 cells and A-431cells. The primary antibody was incubated at 2.0 µg/ml overnight at 4˚C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Cell Profiling group, SciLifeLab Stockholm.
In a sandwich ELISA (assuming 100µl/well), a concentration of 4.0-8.0 µg/ml of this antibody will detect at least 1000 pg/ml of recombinant human SCF when used with BioGems' biotinylated antigen affinity purified anti-human SCF (60-291BT) as the detection antibody at a concentration of approximately 1.0-2.0 µg/ml.
To detect hSCF by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSCF is 2.0-4.0 ng/lane, under non-reducing conditions.