Creating Value for Life Science Research!


Get a Free Sample of this product!
shopping cart image

Anti-Human PDGF-BB



Catalog Number : 500-P47

Description

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hPDGF-BB. Anti-Human PDGF-BB specific antibody was purified by affinity chromatography employing immobilized hPDGF-BB matrix.

Additional Information

Immunogen
Recombinant Human PDGF-BB (BioGems Catalog #100-14B)
 
Clone:
Polyclonal Rabbit
 
Format:
purified
 
Applications:
Immunohistochemistery:
This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentration is 1.0 μg/ml overnight at 4°C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary.
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hPDGF-BB (2.5 ng/ml), a concentration of 0.03-0.04 µg/ml of this antibody is required.
Sandwich ELISA:
To detect hPDGF-BB by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human PDGF-BB (60-267BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPDGF-BB.
Western Blot:
To detect hPDGF-BB by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-BB is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
 
Reactivity:
Human
 

Availability: In stock

Size Price Qty
50μg
$195.00
100μg
$250.00
1mg
$1,700.00