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Anti-Human β-NGF



Catalog Number : 500-P85

Description

Produced from sera of rabbits pre-immunized with highly pure recombinant Human β-NGF. Anti-Human β-NGF specific antibody was purified by affinity chromatography employing immobilized Human β-NGF matrix.

Additional Information

Immunogen
Recombinant Human β-NGF (BioGems Catalog #450-01)
 
Clone:
Polyclonal Rabbit
 
Format:
purified
 
Applications
Immunohistochemistery:
This antibody stained formalin-fixed, paraffin-embedded sections of human pancreas adenocarcinoma. The recommended concentration is 0.5 mg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results were achieved without antigen retrieval. Optimal concentrations and conditions may vary.
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Human β-NGF (1 ng/ml), a concentration of 0.05-0.08 µg/ml of this antibody is required.
Sandwich ELISA:
To detect Human β-NGF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human β-NGF (60-247BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human β-NGF.
Western Blot:
To detect Human β-NGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human β-NGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
 
Reactivity
Human
 

Availability: In stock

Size Price Qty
50μg
$195.00
100μg
$250.00
1mg
$1,700.00