Anti-Human/Mouse CD11b PE

Catalog Number : 03221-60

Description

The M1/70 monoclonal antibody specifically reacts with the 170 kDa αM integrin chain of mouse CD11b from the Mac-1 integrin (CD11b/CD18). Mac-1 binds to C3bi, CD54 (ICAM-1), and fibrinogen, and it is expressed by granulocytes, macrophages, NK cells, myeloid-derived dendritic cells, microglia, activated lymphocytes, and mouse B-1 cells. The expression is up-regulated on activated neutrophils at the same time that L-selectin is shed from the cell surface. The M1/70 antibody is used for the detection of monocytes, granulocytes, and a subset of natural killer cells in human peripheral blood.

M1/70 blocks C3bi binding and cell adherence, but not cell-mediated lysis and it cross-reacts with human CD11b.

C57Bl/6 bone marrow cells were stained with PE M1/70 with relevant isotype control in Gray

Additional Information

Clone:	M1/70
Format:	PE
Applications:	FC
Reactivity:	Human, Mouse
Isotype:	Rat IgG2b, kappa
Research Interest:	Innate Immunity, Angiogenesis
Cell Type:	Monocytes and Macrophages, NK and NKT Cells
Preparation:	The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Formulation:	Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
References:	Sanchez-Madrid, F., Simon, P., Thompson, S., & Springer, T. A. (1983). Mapping of antigenic and functional epitopes on the alpha-and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1.The Journal of experimental medicine, 158(2), 586-602. Ault, K. A., & Springer, T. A. (1981). Cross-reaction of a rat-anti-mouse phagocyte-specific monoclonal antibody (anti-Mac-1) with human monocytes and natural killer cells. The Journal of Immunology, 126(1), 359-364. Springer, T., Galfre, G., Secher, D. S., & Milstein, C. (1978). Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. European journal of immunology, 8(8), 539-551.

Availability: In stock

Size	Price	Qty
25 μg	$60.00
100 μg	$125.00

Additional M1/70 Formats

Cat.No.	Format	Starting From
03221-60	PE	$ 60.00
03221-65	PE-Cyanine5	$ 65.00
03221-70	PerCP-Cyanine5.5	$ 70.00
03221-20	Purified	$ 90.00
03221-50	FITC	$ 70.00
03221-30	Biotin	$ 30.00
03221-87	APC-Cyanine7	$ 75.00
03221-80	APC	$ 50.00
03221-77	PE-Cyanine7	$ 70.00
03221-40	BG Violet 450	$ 110.00
03221-25	SAFIRE Purified	$ 135.00