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Anti-Human MIA

Catalog Number : 500-P243


Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMIA. Anti-Human MIA specific antibody was purified by affinity chromatography employing immobilized hMIA matrix.

Additional Information

Recombinant Human MIA (BioGems Catalog #130-01)
Polyclonal Rabbit
This antibody stained formalin-fixed paraffin-embedded sections of human pancreas infiltrating ductal adenocarcinoma tissue. The recommended concentration is 1.0 µg/ml - 2.0 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with an alcohol-soluble AEC chromogen. Optimal results for these conditions were achieved with heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer or enzyme induced antigen retrieval with proteinase K at room temperature. Optimal concentrations and conditions may vary.
Sandwich ELISA:
To detect hMIA by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human MIA (60-219BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIA.
Western Blot:
To detect hMIA by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIA is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Availability: In stock

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