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Anti-Human LEC

Catalog Number : 500-M43


Produced in BALB/c x ICR F1 mice using highly pure (>98%) recombinant human LEC as the immunizing antigen. This IgG1K antibody was purified from ascites fluid by Protein G affinity chromatography.

Additional Information

Recombinant Human LEC (CCL16) (BioGems Catalog #300-44)
Monoclonal Mouse
To yield one-half maximal inhibition [ND50] of the biological activity of Human LEC (100 ng/ml), a concentration of 1.0-5.0 μg/ml of this antibody is required.
Sandwich ELISA:
In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect at least 500 pg/ml of recombinant human LEC when used with BioGems' biotinylated antigen affinity purified anti-human LEC (60-203BT) as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml.
Western Blot:
To detect hLEC by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLEC is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.

Availability: In stock

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