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Anti-Human IL-7

Catalog Number : 500-M07


Produced in BALB/c x C57BL/6 F1 mice using highly pure recombinant human IL-7 as the immunizing antigen. This IgG1 antibody was purified from ascites fluid by Protein A affinity chromatography.

Additional Information

Recombinant Human IL-7 (BioGems Catalog #200-07)
Monoclonal Mouse
Sandwich ELISA:
In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect at least 1000 pg/ml of recombinant human IL-7 when used with BioGems' biotinylated antigen affinity purified anti-human IL-7 (60-007BT) as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml.
Western Blot:
To detect hIL-7 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-7 is 2.0-4.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.

Availability: In stock

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