Anti-Human CD28 APC
The CD28.2 monoclonal antibody specifically binds with the human 44 kDa homodimeric trans-membrane glycoprotein CD28, expressed on the surface of most mature T lymphocytes, plasma cells, and thymocytes. CD28 is a ligand for B7-1 (CD80) and B7-2 (CD86), a co-stimulator of T lymphocytes, and enhances the interaction between T and B lymphocytes. It has been reported that the T lymphocytes stimulation to produce IL-2 depends on the monoclonal antibody involved, which suggests that the CD28 molecule presents some subregions with distinct functions. The CD28.2 antibody induces Ca2+ influx in Jurkat T lymphocytes. Other studies have shown that CD28 is involved in the signal transduction.
Mouse IgG1, kappa
|Research Interest:||Adaptive Immunity|
|Cell Type:||T Cells|
|Protein Cross Reactivity:||Baboon, Chimpanzee, Cynomolgus, Rhesus|
The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York
Nunès, J., Klasen, S., Ragueneau, M., Pavon, C., Couez, D., Mawas, C., ... & Olive, D. (1993). CD28 mAbs with distinct binding properties differ in their ability to induce T cell activation: analysis of early and late activation events.International immunology, 5(3), 311-315.
Karlsson, I., Malleret, B., Brochard, P., Delache, B., Calvo, J., Le Grand, R., & Vaslin, B. (2007). FoxP3+ CD25+ CD8+ T-cell induction during primary simian immunodeficiency virus infection in cynomolgus macaques correlates with low CD4+ T-cell activation and high viral load. Journal of virology, 81(24), 13444-13455.