Anti-Human CD14 BG Violet 450
The 61D3 monoclonal antibody specifically reacts with CD14, a cell surface anchored glycoprotein that is primarily expressed on monocytes, inerfollicular macrophages, and a subset of dendritic cells. CD14 associates with MD-2 (LY-96) and TLR4 to form a receptor complex, which signals specifically in response to bacterial lipopolysaccharide (LPS) binding. The CD14/MD-2/TLR4 receptor complex signals via MyD88, TIRAP, and TRAF6, and ultimately activates NF-kappaB.
BG Violet 450 conjugate is an alternative to the Pacific Blue, eFluor 450, or BD Horizon V450 dyes. It is excited by the violet (405 nm) laser, with a peak emission of 450nm.
BG Violet 450
Mouse IgG1, kappa
|Research Interest:||Innate Immunity|
|Cell Type:||Macrophage / Monocyte|
The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
Schlossman, S. F. (1995). Leucocyte typing V: White cell differentiation antigens: Proceedings of the Fifth International Workshop and Conference, Held in Boston, USA 3-7 November, 1993. Oxford University Press.
Leucocyte Typing VI: White Cell Differentiation Antigens: Proceedings of the Sixth International Workshop and Conference Held in Kobe, Japan, 10-14 November 1996. Garland Pub., 1998.
Devitt, A., Moffatt, O. D., Raykundalia, C., Capra, J. D., Simmons, D. L., & Gregory, C. D. (1998). Human CD14 mediates recognition and phagocytosis of apoptotic cells. Nature, 392(6675), 505-509.