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Anti-Human BDNF



Catalog Number : 500-P84

Description

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBDNF. Anti-Human BDNF specific antibody was purified by affinity chromatography employing immobilized hBDNF matrix.

Additional Information

Immunogen
Recombinant Human BDNF (BioGems Catalog #450-02)
 
Clone:
Polyclonal Rabbit
 
Format:
purified
 
Applications:
Immunohistochemistery:
This antibody stained U-2 OS and U-251 MG cells.  The primary antibody was incubated at 2.0 µg/ml overnight at 4°C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Cell Profiling group, SciLifeLab Stockholm.
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hBDNF (2.0 μg/ml), a concentration of 0.54-0.81 μg/ml of this antibody is required.
Sandwich ELISA:
To detect hBDNF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human BDNF (60-080BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBDNF.
Western Blot:
To detect hBDNF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBDNF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
 
Reactivity:
 

Availability: In stock

Size Price Qty
50μg
$195.00
100μg
$250.00
1mg
$1,700.00