Rat IL-4 Pre-Coated ELISA Kit

Grouped product items
Size Price Qty
1 Kit
$490.00
Catalog Number : BGK20096

description

IL-4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. Produced by mast cells, T cells and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy.

The BioGems Rat IL-4 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure IL-4 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for IL-4. It contains E. coli-expressed recombinant IL-4. The detection antibody is a biotinylated goat polyclonal antibody specific for IL-4. The kit is analytically validated with ready to use reagents.

Product Details

Assay Type:
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:
<1pg/ml
Range:
15.6pg/ml-1000pg/ml
Specificity:
Natural and recombinant Rat IL-4
Uniprot:
P20096
Interference:
No detectable cross-reactivity with other relevant proteins
Storage Conditions:
Store the unopened product at 2 - 8 °C.

Components

Description

Quantity

Volume

Anti-Rat IL-4 Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Rat IL-4 Standard

2

10 ng vial

Rat IL-4 Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

54.7

428

738

53.8

387

729

Standard Deviation

3.23

21.83

31.73

4.79

25.93

45.2

Consistency (%CV)

5.9

5.1

4.3

8.9

6.7

6.2

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