Murine TNF-α Pre-Coated ELISA Kit

TNF-α is a pleiotropic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-α is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-α also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation, and diabetes.

The BioGems Murine TNF-α Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Murine TNF-α with a 96-well strip plate that is pre-coated with rat monoclonal antibody specific for TNF-α. It contains E. coli-expressed recombinant Murine TNF-α. The detection antibody is a biotinylated goat polyclonal antibody specific for TNF-α. The kit is analytically validated with ready to use reagents.