Human IL-13 Pre-Coated ELISA Kit
description
IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and also by mast cells and NK cells. Targeted deletion of IL-13 in mice resulted in impaired Th2 cell development and indicated an important role for IL-13 in the expulsion of gastrointestinal parasites. IL-13 exerts anti-inflammatory effects on monocytes and macrophages and it inhibits the expression of inflammatory cytokines such as IL-1β, TNF-α, IL-6 and IL-8. IL-13 has also been shown to enhance B cell proliferation and to induce isotype switching, resulting in increased production of IgE. Blocking of IL-13 activity inhibits the pathophysiology of asthma. Human and murine IL-13 are cross-species reactive.
The BioGems Human IL-13 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human IL-13 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for IL-13. It contains E. coli-expressed recombinant Human IL-13. The detection antibody is a biotinylated goat polyclonal antibody specific for IL-13. The kit is analytically validated with ready to use reagents.
Product Details
Assay Type:
|
Solid Phase Sandwich ELISA: 96-Well Strip Plate
|
Sensitivity:
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<10 pg/ml
|
Range:
|
15.6 pg/mL- 1,000 pg/mL
|
Specificity:
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Natural and recombinant Human IL-13
|
Uniprot:
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P35225
|
Interference:
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No detectable cross-reactivity with other relevant proteins
|
Storage Conditions:
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Store the unopened product at 2 - 8 °C.
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Components
Description |
Quantity |
Volume |
Anti-Human IL-13 Pre-coated 96-well strip microplate |
1 |
12 strips of 8 wells |
Human IL-13 Standard |
2 |
10 ng vial |
Human IL-13 Biotinylated antibody (100x) |
1 |
130 µL |
Avidin-Biotin-Peroxidase Complex (100x) |
1 |
130 µL |
Sample Diluent |
1 |
30mL |
Antibody Diluent |
1 |
12mL |
Avidin-Biotin-Peroxidase Diluent |
1 |
12mL |
Wash Buffer Concentrate (Powder for 1000mL) |
1 |
Pack |
Color Developing Reagent |
1 |
10mL |
Stop Solution |
1 |
10mL |
Plate Sealers |
4 |
Each |
Reproducibility
|
Intra-Assay |
Inter-Assay |
||||
Sample ID |
1 |
2 |
3 |
1 |
2 |
3 |
n= |
16 |
16 |
16 |
24 |
24 |
24 |
Mean Measured Concentration (pg/mL) |
98 |
340 |
497 |
115 |
354 |
512 |
Standard Deviation |
4.02 |
10.88 |
19.38 |
8.28 |
19.82 |
29.7 |
Consistency (%CV) |
4.1 |
3.2 |
3.9 |
7.2 |
5.6 |
5.8 |