Human IL-1β Pre-Coated ELISA Kit
description
IL-1β is a proinflammatory cytokine produced in a variety of cells, including monocytes, tissue macrophages, keratinocytes, and other epithelial cells. Both IL-1α and IL-1β bind to the same receptor and have similar, if not identical, biological properties. These cytokines have a broad range of activities including the stimulation of thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity, and the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1β is a secreted cytokine, IL-1α is predominantly a cell-associated cytokine.
The BioGems Human IL-1β Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure IL-1&beta with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for IL-1&beta. It contains E. coli-expressed recombinant Human IL-1&beta. The detection antibody is a biotinylated goat polyclonal antibody specific for IL-1&beta. The kit is analytically validated with ready to use reagents.Product Details
Assay Type:
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Solid Phase Sandwich ELISA: 96-Well Strip Plate
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Sensitivity:
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<.15 pg/ml
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Range:
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3.9 pg/mL- 250 pg/mL
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Specificity:
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Natural and recombinant Human IL-1β
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Uniprot:
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P01584
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Interference:
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No detectable cross-reactivity with other relevant proteins
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Storage Conditions:
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Store the unopened product at 2 - 8 °C.
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Components
Description |
Quantity |
Volume |
Anti-Human IL-1β Pre-coated 96-well strip microplate |
1 |
12 strips of 8 wells |
Human IL-1β Standard |
2 |
1 ng vial |
Human IL-1β Biotinylated antibody (100x) |
1 |
130 µL |
Avidin-Biotin-Peroxidase Complex (100x) |
1 |
130 µL |
Sample Diluent |
1 |
30mL |
Antibody Diluent |
1 |
12mL |
Avidin-Biotin-Peroxidase Diluent |
1 |
12mL |
Wash Buffer Concentrate (Powder for 1000mL) |
1 |
Pack |
Color Developing Reagent |
1 |
10mL |
Stop Solution |
1 |
10mL |
Plate Sealers |
4 |
Each |
Reproducibility
|
Intra-Assay |
Inter-Assay |
||||
Sample ID |
1 |
2 |
3 |
1 |
2 |
3 |
n= |
16 |
16 |
16 |
24 |
24 |
24 |
Mean Measured Concentration (pg/mL) |
19 |
41.2 |
116 |
27.5 |
61.3 |
179.1 |
Standard Deviation |
1 |
2.6 |
6.5 |
1.6 |
4.1 |
12.7 |
Consistency (%CV) |
5.3 |
6.3 |
5.6 |
5.8 |
6.7 |
7.1 |