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Anti-Mouse TCR beta BG Violet 450



Catalog Number : 89612-40

Description

The H57-597 monoclonal antibody is specific for the beta chain of the mouse T cell Receptor (TCR). The crosslinking induces activation and proliferation of T cells, as a plate-bound or a soluble H57-597, or the plate-bound antibody can induce apoptosis, based on assay conditions. The beta chain of the TCR can combine with the alpha chain of the receptor to produce the alpha-beta TCR, which is expressed by the NKT cells, by the NK1.1+ thymocytes and most of the T cells. The beta chain does not react with the gamma-delta TCR-bearing cells, expressed by a small number of T cells.

This antibody can be used as a phenotypic marker for the TCR beta expressing cells or for the functional purpose of TCR-mediated cell activation or apoptosis.

BG Violet 450 conjugate is an alternative to the Pacific Blue, eFluor 450, or BD Horizon V450 dyes. It is excited by the violet (405 nm) laser, with a peak emission of 450nm.

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C57Bl/6 splenocytes were stained with BG Violet 450 H57-597 with relevant isotype control in Red.
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Additional Information

Clone
H57-597
Format:
BG Violet 450
Applications
FC
Reactivity
Mouse
Isotype:
Armenian Hamster IgG
Research Interest: Adaptive Immunity
Cell Type: T Cells
Preperation:
The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Formulation:
Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
References:

Kubo, R. T., Born, W., Kappler, J. W., Marrack, P., & Pigeon, M. (1989). Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors. The Journal of Immunology142(8), 2736-2742.

Gascoigne, N. R. (1990). Transport and secretion of truncated T cell receptor beta-chain occurs in the absence of association with CD3. Journal of Biological Chemistry265(16), 9296-9301.

Kruisbeek, A. M., Shevach, E., & Thornton, A. M. (2004). Proliferative assays for T cell function. Current Protocols in Immunology, 3-12.

Saint-Ruf, C., Panigada, M., Azogui, O., Debey, P., von Boehmer, H., & Grassi, F. (2000). Different initiation of pre-TCR and γδTCR signalling. Nature,406(6795), 524-527.


Availability: In stock

Size Price Qty
25 μg
$80.00
100 μg
$175.00
Additional H57-597 Formats
Cat.No. Format Starting From
89612-70PerCP-Cyanine5.5 $ 85.00
89612-40BG Violet 450 $ 80.00
89612-20Purified $ 80.00
89612-50FITC $ 35.00
89612-80APC $ 65.00