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Anti-Mouse CD45.2 FITC



Catalog Number : 07532-50

Description

The 104 monoclonal antibody specifically reacts with the mouse CD45 molecule, the Leukocyte Common Antigen (LCA) which occurs in the alloantigen CD45.2-expressing mouse strains (C57BL/6, CBA, 129, A, AKR, C58, DBA/1, DBA/2, BALB/c, and C3H/He). The 104 monoclonal antibody does not react with the leukocytes of the CD45.1-expressing mouse strains (DA, SJL/J, RIII, and STS/A).

The CD45 molecule is a member of the Protein Tyrosine Phosphatase (PTP) family, because its intracellular region contains two PTP domains. The extracellular region’s variability is caused by different levels of glycosylation, and the splicing of the 4, 5, and 6 exons.

The isoforms found in the mouse strains depend on the activation state, maturation stage and cell type, and are very important in B and T lymphocytes antigen receptor signal transduction. The 104 antibody inhibits some of the B lymphocytes responses, reduces the serum IgG levels, and influences the autoimmune renal pathology.

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C57BI/6 bone marrow cells were stained with FITC 104 with relevant isotype control in Red.
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Additional Information

Clone
104
Format:
FITC
Applications
FC
Reactivity
Mouse
Isotype:
Mouse IgG2a, kappa
Research Interest: Adaptive Immunity
Cell Type: B Cells, Monocytes and Macrophages, NK and NKT Cells, T Cells
Preperation:
The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Formulation:
Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
References:

Shen, F. W., Tung, J. S., & Boyse, E. A. (1986). Further definition of the Ly-5 system. Immunogenetics24(3), 146-149.

Shen, F.W. (1981) Monoclonal antibodies to mouse lymphocyte differentiation alloantigens. Monoclonal Antibodies and T-Cell Hybridomas: Perspectives and Technical Advances. G.J. Hämmerling, U. Hämmerling, and J.F. Kearney, editors. Elsevier/North-Holland, Amsterdam. pp. 25–31.

Yakura, H., Shen, F. W., Bourcet, E., & Boyse, E. A. (1983). On the function of Ly-5 in the regulation of antigen-driven B cell differentiation. Comparison and contrast with Lyb-2. The Journal of experimental medicine157(4), 1077-1088.

Suzuki, K., Oida, T., Hamada, H., Hitotsumatsu, O., Watanabe, M., Hibi, T., ... & Ishikawa, H. (2000). Gut cryptopatches: direct evidence of extrathymic anatomical sites for intestinal T lymphopoiesis. Immunity13(5), 691-702.


Availability: In stock

Size Price Qty
25 μg
$40.00
100 μg
$125.00
500 μg
$175.00
Additional 104 Formats
Cat.No. Format Starting From
07532-60PE $ 60.00
07532-70PerCP-Cyanine5.5 $ 65.00
07532-40BG Violet 450 $ 75.00
07532-20Purified $ 80.00
07532-50FITC $ 40.00
07532-87APC-Cyanine7 $ 95.00
07532-80APC $ 55.00
07532-77PE-Cyanine7 $ 55.00