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Anti-Mouse CD3e SAFIRE Purified



Catalog Number : 05122-25

Description

The 145-2C11 monoclonal antibody reacts with mouse CD3e, the 20 kDa ε chain of the TCR complex. Together with the γ and δ subunits of CD3, the ε subunits are involved in the assembly, trafficking, and surface expression of T-cell receptor complex. CD3 is expressed on thymocytes, mature T cells, and natural killer T cells, and the ε chain enhances the antigen recognition.

The 145-2C11 antibody binds to the TCR complex and, depending on the conditions, initiates T cells activation, proliferation, and apoptosis. The soluble antibody seems to block lysis of target cells by antigen-specific cytotoxic T lymphocytes.

The 145-2C11 antibody does not cross-react with the rat leukocytes, and it is used as a phenotypic marker for mouse T lymphocytes.

Additional Information

Clone
145-2C11
Format:
SAFIRE Purified
Applications
FC, FA, IHC, IF, IP, WB
Reactivity
Mouse
Isotype:
Armenian Hamster IgG
Research Interest: Adaptive Immunity
Cell Type: NK and NKT Cells, T Cells, Treg cells, Th17 cells
Preperation:
The product should be stored undiluted at 4°C. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography. The endotoxin level is determined by LAL test to be less than 0.01 EU/µg of the protein.
Formulation:
Phosphate-buffered aqueous solution, ph7.2.
References:

Leo, O., Foo, M., Sachs, D. H., Samelson, L. E., & Bluestone, J. A. (1987). Identification of a monoclonal antibody specific for a murine T3 polypeptide.Proceedings of the National Academy of Sciences84(5), 1374-1378.

Nakano, H., Yamazaki, T., Miyatake, S., Nozaki, N., Kikuchi, A., & Saito, T. (1996). Specific Interaction of Topoisomerase II and the CD3 Chain of the T Cell Receptor Complex. Journal of Biological Chemistry271(11), 6483-6489.

Salmond, R. J., Filby, A., Pirinen, N., Magee, A. I., & Zamoyska, R. (2011). Mislocalization of Lck impairs thymocyte differentiation and can promote development of thymomas. Blood117(1), 108-117.


Availability: In stock

Size Price Qty
100 μg
$105.00
500 μg
$180.00
1 mg
$335.00
Additional 145-2C11 Formats
Cat.No. Format Starting From
05122-60PE $ 30.00
05122-65PE-Cyanine5 $ 65.00
05122-70PerCP-Cyanine5.5 $ 70.00
05122-77PE-Cyanine7 $ 70.00
05122-20Purified $ 65.00
05122-25SAFIRE Purified $ 105.00
05122-50FITC $ 25.00
05122-30Biotin $ 35.00
05122-80APC $ 55.00