Creating Value for Life Science Research!


Get a Free Sample of this product!
shopping cart image

Anti-Mouse CD3 BG Violet 450



Catalog Number : 05112-40

Description

The 17A2 monoclonal antibody specifically reacts with the mouse T lymphocytes receptor (TCR) associated CD3 complex, resulting in cellular activation and proliferation. CD3 is expressed by thymocytes and mature lymphocytes, and contains γ, δ, and ε subunits, involved in the assembly, trafficking, and surface expression of T-cell receptor complex.

The interaction between the T lymphocytes and the 17A2 antibody can be blocked by the 145-2C11 anti-CD3e antibody, demonstrating that the 17A2 antibody recognizes the CD3 ε chain.

BG Violet 450 conjugate is an alternative to the Pacific Blue, eFluor 450, or BD Horizon V450 dyes. It is excited by the violet (405 nm) laser, with a peak emission of 450nm.

product image
C57Bl/6 splenocytes were stained with BG Violet 450 17A2 with relevant isotype control in Red.
product image

Additional Information

Clone
17A2
Format:
BG Violet 450
Applications
FC
Reactivity
Mouse
Isotype:
Rat IgG2b, kappa
Research Interest: Adaptive Immunity
Cell Type: NK and NKT Cells, T Cells, Treg cells, Th17 cells
Preperation:
The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Formulation:
Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
References:

Miescher, G. C., Schreyer, M., & MacDonald, H. R. (1989). Production and characterization of a rat monoclonal antibody against the murine CD3 molecular complex. Immunology letters23(2), 113-118.

Mysliwietz, J., & Thierfelder, S. (1992). Antilymphocytic antibodies and marrow transplantation. XII. Suppression of graft-versus-host disease by T-cell-modulating and depleting antimouse CD3 antibody is most effective when preinjected in the marrow recipient. Blood80(10), 2661-2667.

Wu, L., Antica, M., Johnson, G. R., Scollay, R., & Shortman, K. (1991). Developmental potential of the earliest precursor cells from the adult mouse thymus. The Journal of experimental medicine174(6), 1617-1627.


Availability: In stock

Size Price Qty
25 μg
$75.00
100 μg
$175.00
Additional 17A2 Formats
Cat.No. Format Starting From
05112-87APC-Cyanine7 $ 85.00
05112-40BG Violet 450 $ 75.00
05112-20Purified $ 20.00
05112-25SAFIRE Purified $ 105.00
05112-60PE $ 55.00
05112-80APC $ 60.00
05112-50FITC $ 25.00