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Anti-Human c-Myc Purified



Catalog Number : 72211-20

Description

The 9E10 monoclonal antibody specifically reacts with the human c-Myc p67 molecule, a proto-oncogene from the Myc family, which is important in transformation, proliferation, and differentiation. This gene is expressed during embryonic development, in some adult tissues, and is amplified in some tumors. Inside the cell, the gene is localized in the nucleus or cytoplasm. C-Myc is characterized by a Leucine zipper, a basic region, and a helix-loop-helix, which allow the formation of a heterodimer Myc-Max that binds to DNA, and activates the transcription.

The 9E10 antibody recognizes the human c-Myc and can be used for Myc-tagged protein detection. It was obtained by using synthetic peptide similar to the terminal domain of human c-Myc as an immunogen.

Additional Information

Clone
9E10
Format:
Purified
Applications
FC, IF, ICC, IP, WB
Reactivity
Human
Isotype:
Mouse IgG1, kappa
Preperation:
The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Formulation:
Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
References:

Campbell, A. M., Kessler, P. D., & Fambrough, D. M. (1992). The alternative carboxyl termini of avian cardiac and brain sarcoplasmic reticulum/endoplasmic reticulum Ca (2+)-ATPases are on opposite sides of the membrane. Journal of Biological Chemistry267(13), 9321-9325.

Hilpert, K., Hansen, G., Wessner, H., Küttner, G., Welfle, K., Seifert, M., & Höhne, W. (2001). Anti-c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose. Protein engineering14(10), 803-806.

Krauß, N., Wessner, H., Welfle, K., Welfle, H., Scholz, C., Seifert, M., ... & Höhne, W. (2008). The structure of the anti-c-myc antibody 9E10 Fab fragment/epitope peptide complex reveals a novel binding mode dominated by the heavy chain hypervariable loops. Proteins: Structure, Function, and Bioinformatics73(3), 552-565.


Availability: In stock

Size Price Qty
25 μg
$75.00
100 μg
$165.00
Additional 9E10 Formats
Cat.No. Format Starting From
72211-20Purified $ 75.00